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KMID : 1007520080170051092
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Food Science and Biotechnology
2008 Volume.17 No. 5 p.1092 ~ p.1096
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Presence of Transgenic Genes and Proteins in Commercial Soybean Foods from Mexican Grocery Stores
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Yendi Arely Cruz-Flores
Raul Rodriguez-Herrera
Cristobal Noe Aguilar-Gonzalez
Juan Carlos Contreras-Esquivel
Reyes-Vega Maria Luz
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Abstract
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Commercial food products from major cities of Coahuila, Mexico were screened to identify residues of transgenic deoxyribonucleic acid (DNA) and/or proteins. After performed, an inventory on all products that contained a soybean-based ingredient in a commercial grocery store in the city of Saltillo, Coahuila, Mexico, 245 food products were identified and grouped in 15 classes according to the soybean ingredient as well as the manufacturing process used for their elaboration. Similar sampling was made for the different food classes in the cities of Monclova, Piedras Negras, and Torreon. A total of 88 samples were analyzed and DNA was extracted by the hexadecyltrimethyl-ammonium bromide (CTAB) technique with slight modification to obtain better DNA quality (1). In addition, segments of the transgenic genes one that codifies for 5-enolpyruvylshikimate-3-phosphate synthase (epsps), cry 1A, and the cauliflower mosaic virus (CaMV) promoter were amplified using polymerase chain reaction (PCR). The transgenic proteins 5- nolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS) and insecticidal crystal protein (Cry 1Ab/Ac) were identified using double antibody sandwich-enzymatic linked immunoassay analysis (DAS-ELISA). Presence of transgenic genes and/or proteins was identified in 35.3% of the commercial products samples.
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KEYWORD
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cry 1AB, epsps gene, 35s cauliflower mosaic virus (CaMV) promoter, transgenic proteins 5-enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS), insecticidal crystal protein (Cry 1Ab/Ac)
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